@article{DI2025, 
author = {Yumin DI and Jing CHANG and Hua MA and Nan XIAO and Bo ZOU and Qinghua LIU and Xinxin ZHOU and Changhai LI and Kai ZHANG and Shichao RUAN and Ruochen YANG},
title = {Determination of Etomidate in Blood and Hair by QTRAP-LC-MS/MS},
year = {2025},
journal = {Forensic Science and Technology},
volume = {50},
number = {4},
pages = {379-385},
keywords = {forensic toxicology, LC-MS/MS, MRM-IDA-EPI, hair analysis, etomidate},
url = {https://www.sciopen.com/article/10.16467/j.1008-3650.2024.0081},
doi = {10.16467/j.1008-3650.2024.0081},
abstract = {To establish a high performance liquid chromatography-tandem hybrid triple-quadrupole linear ion trap mass-spectrometry method for qualitative and quantitative determination of etomidate in blood and hair. Deuterium cocaine was selected as the internal standard, and blood sample was extracted by acetonitrile (V/V, 1/6), then centrifuged at high speed; hair sample (about 20 mg) was ground by a ball mill, then extracted by methanol and passed through the organic membrane. Using 0.1% (V/V) formic acid aqueous solution and 0.1% (V/V) formic acid acetonitrile as mobile phase, the analyte was separated and analyzed by an ACQUITY UPLC®C18 (2.1 mm×100 mm×1.7 μm) column. Electrospray positive multiple reaction monitoring/information-dependent acquisition/enhanced product ion scanning (MRM-IDA-EPI) and secondary library retrieval were used for analysis. The results showed that etomidate in blood had a good linear relationship in the range of 1.0 to 100.0 ng/mL (r > 0.995). The linear relationship of etomidate in hair was good in the range of 0.05 to 5.0 ng/mg (r > 0.995). The detection limits (S/N ≥ 3) for blood and hair were 0.2 ng/mL and 0.002 ng/mg, and the quantitative limits (S/N ≥ 10) were 0.5 ng/mL and 0.005 ng/mg respectively. The recoveries of 1.0, 10.0, 100.0 ng/mL in spiked blood were 97.1% to 103.4%, and 0.5, 2.5, 5.0 ng/mg in spiked hair were 84.0% to 99.8%. The relative standard deviation was less than 15%. This method can be applied to the qualitative and quantitative analysis of etomidate in blood and hair in the juridical practice.}
}