@article{Xiang2026, 
author = {Wen Xiang and Mingrui Yang and Longfei Ding and Panyu Yi and Yingjian Zhang and Yangping Chen and Yuan Yao and Yunfeng Jiang and Ming Hao and Lin Huang and Chaolan Fan and Shunzong Ning and Lianquan Zhang and Bo Jiang and Xuejiao Chen and Xue Chen and Dengcai Liu and Zhongwei Yuan},
title = {Mapping and introgression of all-stage stripe rust resistance gene Yr1T from Aegilops mutica into common wheat using a dominant Ph1-suppression system},
year = {2026},
journal = {The Crop Journal},
volume = {14},
number = {4},
pages = {1277-1286},
keywords = {Yellow rust, Homoeologous pairing, Alien gene transfer, Chromosome engineering, Gene mapping},
url = {https://www.sciopen.com/article/10.1016/j.cj.2026.02.011},
doi = {10.1016/j.cj.2026.02.011},
abstract = {Aegilops mutica (genome T) harbors stripe rust resistance and is a potentially valuable genetic resource for broadening the genetic basis of disease resistance in common wheat. A dominant Ph1-suppression system in Ae. mutica promotes homoeologous pairing and recombination between wheat and alien chromosomes even in the presence of Ph1, providing a tool for alien gene mapping and introgression. In this study, we identified an all-stage stripe rust resistance gene, Yr1T, in Ae. mutica and mapped it to chromosome 1T using chromosome deletion lines of Langdon × Ae. mutica amphidiploids. Genetic stocks with an AABBTD genomic constitution were generated by crossing resistant amphidiploids with the susceptible common wheat cultivar MX169. We induced homoeologous recombination between chromosome 1T and wheat homoeologous group 1 chromosomes by leveraging the Ae. mutica Ph1-suppression system. Integrating cytological identification, molecular markers, and RNA-seq breakpoint mapping, Yr1T was delimited to an approximately 20 Mb physical interval near the centromere in chromosome arm 1TS. Screening of a 100-plant F2 population yielded six recombinant plants with different breakpoints (6% recombination frequency), and a translocation line carrying Yr1T was developed. Collectively, these results demonstrated the high efficiency of the Ae. mutica Ph1-suppression system for alien gene mapping and targeted transfer, providing a practical reference for its application in wheat breeding.}
}