@article{Guo2026, 
author = {Conghui Guo and Guangbin Liu and Jie Liu and Kaihao Chen and Ming Deng and Baoli Sun and Yongqing Guo and Dewu Liu and Yaokun Li},
title = {scRNA-seq reveals the molecular atlas of the goat follicular microenvironment over the time course of ovulation},
year = {2026},
journal = {Journal of Integrative Agriculture (JIA)},
volume = {25},
number = {9},
pages = {3800-3813},
keywords = {hCG, ovulation, granulosa cell, macrophages},
url = {https://www.sciopen.com/article/10.1016/j.jia.2024.09.029},
doi = {10.1016/j.jia.2024.09.029},
abstract = {Ovulation is paramount for female animal fertility, necessitating a thorough understanding of its process and molecular underpinnings. This study aimed to delineate the temporal dynamics of ovulation in the goat ovary. Utilizing single-cell sequencing, we analyzed follicular fluid samples obtained at 0, 6, 12, 18, and 24 h post-hCG administration, identifying 4 cell types and 6 myeloid cell subtypes. We elucidated gene expression and functional changes in granulosa cells (GCs) over the time course of ovulation. Notably, our study detected and confirmed immune cell infiltration at 6 h post-luteinizing hormone (LH) peak. Additionally, cell–cell communication analysis revealed strong predicted interactions between GCs and macrophages, involving signaling programs associated with immune-cell recruitment, extracellular-matrix remodeling, and oocyte maturation. Collectively, our investigation has established a comprehensive single-cell transcriptome atlas of the ovulatory goat follicular microenvironment for advancing exploration into ovulation mechanisms and developing therapies for ovulatory disorders.}
}