TY  - JOUR
AU  - Guo, Conghui
AU  - Liu, Guangbin
AU  - Liu, Jie
AU  - Chen, Kaihao
AU  - Deng, Ming
AU  - Sun, Baoli
AU  - Guo, Yongqing
AU  - Liu, Dewu
AU  - Li, Yaokun
PY  - 2026
TI  - scRNA-seq reveals the molecular atlas of the goat follicular microenvironment over the time course of ovulation
JO  - Journal of Integrative Agriculture (JIA)
SN  - 2095-3119
SP  - 3800
EP  - 3813
VL  - 25
IS  - 9
AB  - Ovulation is paramount for female animal fertility, necessitating a thorough understanding of its process and molecular underpinnings. This study aimed to delineate the temporal dynamics of ovulation in the goat ovary. Utilizing single-cell sequencing, we analyzed follicular fluid samples obtained at 0, 6, 12, 18, and 24 h post-hCG administration, identifying 4 cell types and 6 myeloid cell subtypes. We elucidated gene expression and functional changes in granulosa cells (GCs) over the time course of ovulation. Notably, our study detected and confirmed immune cell infiltration at 6 h post-luteinizing hormone (LH) peak. Additionally, cell–cell communication analysis revealed strong predicted interactions between GCs and macrophages, involving signaling programs associated with immune-cell recruitment, extracellular-matrix remodeling, and oocyte maturation. Collectively, our investigation has established a comprehensive single-cell transcriptome atlas of the ovulatory goat follicular microenvironment for advancing exploration into ovulation mechanisms and developing therapies for ovulatory disorders.
UR  - https://doi.org/10.1016/j.jia.2024.09.029
DO  - 10.1016/j.jia.2024.09.029