TY  - JOUR
AU  - Li, Xuyan
AU  - Wang, Shouwen
AU  - Li, Hongxue
AU  - Qian, Fangyun
AU  - Wang, Haiyang
AU  - Wang, Jingying
AU  - Zhai, Lulu
AU  - Shi, Baosheng
AU  - Huo, Junwei
AU  - Bian, Shaomin
PY  - 2026
TI  - Transcriptome and metabolome analysis reveals regulatory network associated with light-independent anthocyanin biosynthesis in blueberry fruit
JO  - Horticultural Plant Journal
SN  - 2095-9885
SP  - 584
EP  - 602
VL  - 12
IS  - 3
AB  - Anthocyanins are vital secondary metabolites contributing to fruit pigmentation and antioxidative properties. While light is a well-known regulator of anthocyanin biosynthesis, the molecular basis of light-independent anthocyanin accumulation remains underexplored. In this study, integrated analysis of metabolome and transcriptome showed that the anthocyanin content in blueberry (Vaccinium corymbosum ‘Bluetta’) fruit was slightly decreased by light-impermeable bagging treatment, while anthocyanin biosynthetic genes were transcriptionally inhibited to different levels, suggesting a slight influence of the bagging treatment on anthocyanin accumulation. Further observation showed that fruit bagging did not alter ethylene production but decreased ABA content. Noticeably, two VcMYBA/MYB1s were not transcriptionally altered by the light-impermeable bagging treatment. Consistently, histochemical GUS analysis and pharmacological manipulation suggested light-independent and ethylene-inducible expression of VcMYBA/MYB1. Moreover, WGCNA analysis revealed 3759 genes positively associated with MYBA/MYB1 such as ethylene-associated genes, etc. Additionally, VcbZIP55s and VcCOP1s were activated and inactivated by the bagging treatment, respectively. These findings provided a framework of light-independent anthocyanin biosynthesis in blueberry fruit.
UR  - https://doi.org/10.1016/j.hpj.2025.02.004
DO  - 10.1016/j.hpj.2025.02.004