@article{Lee2026, 
author = {Ji-Eun Lee and Jae Hoon Lee and Dong Uk Ahn and Kee-Tae Kim and Hyun-Dong Paik},
title = {Antioxidative and neuroprotective effects of phosvitin phosphopeptides against H2O2-induced oxidative stress in SH-SY5Y cells},
year = {2026},
journal = {Food Science of Animal Products},
volume = {4},
number = {3},
pages = {9240169},
keywords = {phosvitin phosphopeptides, antioxidant activity, neuroblastoma cell, protective effect, anti-apoptotic properties},
url = {https://www.sciopen.com/article/10.26599/FSAP.2026.9240169},
doi = {10.26599/FSAP.2026.9240169},
abstract = {The antioxidant and protective effects of phosvitin phosphopeptides (PPPs) in human neuroblastoma were studied. Phosvitin from chicken egg yolk was pretreated using high-temperature mild pressure (HTMP) conditions and then hydrolyzed using trypsin (T), Multifect 14L (M), and trypsin + Multifect 14L (TM) to prepare 4 PPPs treatments (HTMP, HTMP-T, HTMP-M, and HTMP-TM). The antioxidant and protective properties of PPPs were assessed using chemical analyses and human neuroblastoma (SH-SY-5Y) cells. All PPPs exhibited reducing power and radical scavenging, metal-chelating, β-carotene bleaching inhibitory, and lipid peroxidation inhibitory activities, but HTMP-TM had the highest antioxidant activity. HTMP-TM increased cell viability and reduced reactive oxygen species production and apoptosis in stressed (H2O2-treated) SH-SY-5Y cells. Additionally, HTMP-TM downregulated B-cell lymphoma-2 (Bcl-2)-associated X protein/Bcl-2 and caspase-3 expression but upregulated catalase expression in oxidatively stressed SH-SY-5Y cells. It was concluded that HTMP-TM exerted protective effects through their antioxidative and anti-apoptotic properties in oxidatively stressed SH-SY-5Y cells.}
}