@article{LUO2005, 
author = {Hui LUO and Huimin YU and Qiang LI and Zhongyao SHEN},
title = {Rapid Cloning and Expression of Glutaryl-7-Aminocephalosporanic Acid Acylase Genes from Soil Samples},
year = {2005},
journal = {Tsinghua Science and Technology},
volume = {10},
number = {5},
pages = {529-534},
keywords = {soil, polymerase chain reaction, Pseudomonas, glutaryl-7-aminocephalosporanic acid acylase},
url = {https://www.sciopen.com/article/10.1016/S1007-0214(05)70113-9},
doi = {10.1016/S1007-0214(05)70113-9},
abstract = {A polymerase chain reaction (PCR)-based strategy was developed to rapidly obtain the gene encoding for an industrially important enzyme, glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase. Different soil samples were cultured with a Pseudomonas selective medium to enrich specific microorganisms, and then the genomic DNA was extracted to serve as PCR templates. PCR primers for GL-7-ACA acylase gene amplification were designed on the basis of bioinformatics searches and analyses. The method was used to successfully amplify three GL-7-ACA acylase genes from different soil samples. The GL-7-ACA acylase genes were then cloned and overexpressed in Escherichia coli with a relatively high level of 266 unit·L-1.}
}