@article{LIU2005, 
author = {Xizhen LIU and Jian HUANG and Rongfu GAO and Yiqin LI},
title = {Expression of an Exo-β-glucanase Gene Is Specifically Related to Lily Pollen Germination and Pollen Tube Growth},
year = {2005},
journal = {Tsinghua Science and Technology},
volume = {10},
number = {4},
pages = {421-425},
keywords = {pollen tube growth, exo-β-glucanase, lily (Lilium longiflorum)},
url = {https://www.sciopen.com/article/10.1016/S1007-0214(05)70093-6},
doi = {10.1016/S1007-0214(05)70093-6},
abstract = {The present study is expected to provide better understanding of how exo-β-glucanase acts on the biochemo-rheological property of the cell wall, and thus the regulation mechanism of pollen tube growth. A polymerase chain reaction (PCR)-based strategy and cDNA library screening were used to clone an exo-β-glucanase gene (LP-ExoI) from Lilium longiflorum pollen tubes. The LP-ExoI sequence showed a high level of homology to that of reported plant exo-β-glucan hydrolases. Phylogenetic analysis demonstrated that there was close relationship between LP-ExoI and other exo-glucan hydrolases in Poacea plants. Northern analysis indicated that LP-ExoI transcripts stored in pollen grains at low levels; LP-ExoI transcription level enhanced at 2 h after the onset of pollen incubation and remained relatively high during pollen tube elongation. Moreover, the expression of LP-ExoI was undetectable in petals, filaments, stigmas, styles, ovaries, leaves, and stems. These results suggest that LP-ExoI gene is a late pollen gene, specifically contributing to the regulation of lily pollen germination and pollen tube growth.}
}