@article{GUO2026, 
author = {Xingli GUO and Yuanhe WANG and Yanye ZHANG and Pengcheng XU and Ying ZHOU and Jiangmiao HU and Liu YANG},
title = {Structural Characterization and in Vitro Hypoglycemic Activity of Polysaccharides from Dendrobium nobile Lindl.},
year = {2026},
journal = {Food Science},
volume = {47},
number = {10},
pages = {85-96},
keywords = {Dendrobium nobile Lindl., polysaccharides, structural characterization, hypoglycemic activity},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20251024-177},
doi = {10.7506/spkx1002-6630-20251024-177},
abstract = {This study explored the structural characteristics and in vitro hypoglycemic activity of polysaccharides from the stems of Dendrobium nobile Lindl.. Two homogeneous polysaccharides, DNP-6 and DNP-7, were obtained by water extraction followed by alcohol precipitation and subsequent successive anion exchange and gel column chromatography. Their structures were analyzed by high performance liquid chromatography (HPLC), ultraviolet-visible (UV-vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR). The results showed that DNP-6 and DNP-7 were neutral polysaccharides with molecular mass of 2.70 × 103 and 1.23 × 103 Da, containing 90.45% and 93.49% total sugar and 2.28% and 1.29% protein, respectively. They were mainly composed of glucose and mannose, with molar ratios of 6.76:1 and 6.63:1, respectively. The glycosidic bonds were mainly of α-configuration, with a small amount of β-pyranose. One-dimensional and two-dimensional NMR results showed that the main chain composition of DNP-6 and DNP-7 was: →4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-β-D-Manp-(1→4)-α-D-Glcp/β-D-Glcp. In addition, the results of in vitro glucose consumption and insulin resistance tests showed that both polysaccharides had good hypoglycemic activity, and DNP-6 possessed stronger activity than DNP-7.}
}