@article{WANG2026, 
author = {Wei WANG and Ting HUANG and Zehui LI and Qianqian WANG and Weihong MIN},
title = {Screening and Functional Validation of Walnut-Derived Bioactive Peptides for Relieving Constipation},
year = {2026},
journal = {Food Science},
volume = {47},
number = {10},
pages = {190-200},
keywords = {molecular docking, constipation, physicochemical stability, walnut peptide FN-10},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20251117-121},
doi = {10.7506/spkx1002-6630-20251117-121},
abstract = {Peptide identification, molecular docking, and a zebrafish model of constipation were used to screen for peptides with laxative activity from an enzymatic hydrolysate of walnut meal. The most promising peptide, FGGDSTHPFN (FN-10), was further evaluated in a mouse model of constipation, and its physicochemical stability was characterized. The results revealed that 10 bioactive peptides with potential laxative effects were obtained, with FN-10 being the most effective among them. After administering FN-10 to constipated mice, we found that compared with the model group, the time to first black stool excretion of mice was significantly shortened by (46.93 ± 3.40)% and (34.65 ± 6.05)% (P < 0.05) in the low-dose (5 mg/kg mb) and high-dose (30 mg/kg mb) FN-10 groups, respectively. The number of fecal pellets at 5 h increased significantly by (102.78 ± 14.09)% and (43.98 ± 11.16)% (P < 0.05), respectively. The wet mass of feces increased significantly by (105.89 ± 14.23)% and (42.39 ± 15.12)% (P < 0.05), respectively, and the dry mass by (149.03 ± 23.00)% and (55.41 ± 17.22)% (P < 0.05), respectively. Additionally, FN-10 exhibited excellent physicochemical stability. Walnut peptide FN-10 is a natural bioactive peptide with laxative effects. This study provides a theoretical foundation for developing functional peptide products to alleviate constipation.}
}