TY  - JOUR
AU  - JIANG, Donglei
AU  - ZHANG, Sidi
AU  - XU, Yang
AU  - WANG, Ke
AU  - WANG, Lifeng
PY  - 2026
TI  - A 5-Hydroxytryptamine Release-Based Biomimetic Microtissue Sensor for Detecting Creatine Kinase
JO  - Food Science
SN  - 1002-6630
SP  - 333
EP  - 340
VL  - 47
IS  - 9
AB  - Based on the principle of creatine kinase (CK)-induced release of 5-hydroxytryptamine (5-HT) from mast cells, the quantitative analysis of CK was achieved by detecting changes in the oxidation current of 5-HT on the electrode surface. Gold nanoparticles (AuNPs), Fe3O4 nanoparticles and multi-walled carbon nanotube (MWCNT) were used jointly to modify the screen-printed carbon electrode (SPCE). Meanwhile, the composite of mast cells and gelatin methacryloyl (GelMA) was used as bio-ink to prepare a connective tissue model with a network structure through 3D bioprinting, which was then combined with an electrochemical workstation to develop a bionic sensor to detect CK. The sensor demonstrated a linear response to CK concentrations ranging from 0.3 to 5.0 μg/mL, which was fitted to the equation: IDPV = 0.23639lgC + 1.94438 (R2 = 0.993, n = 3). The limit of detection (LOD) was determined to be 0.042 μg/mL. The above results confirmed the feasibility of indirectly quantifying food allergen concentrations by detecting cellular secretion during allergic reactions. This study provides a new strategy for rapid allergen detection.
UR  - https://doi.org/10.7506/spkx1002-6630-20251021-139
DO  - 10.7506/spkx1002-6630-20251021-139