@article{Li2026, 
author = {Huadong Li and Lu Luo and Hui Yang and Xing Li and Yuying Li and Haiyang Yu and Kun Zhang and Yuanjie Dong and Benjun Hou and Penghui Miao and Yongshan Wan and Fengzhen Liu},
title = {Fine mapping of the major QTL qLLS.A02.1 and identification of a novel NBS-LRR gene cluster associated with late leaf spot resistance in peanut (Arachis hypogaea L.)},
year = {2026},
journal = {The Crop Journal},
volume = {14},
number = {2},
pages = {547-555},
keywords = {Peanut, Late leaf spot, Whole-genome resequencing, Quantitative trait loci, Fine mapping},
url = {https://www.sciopen.com/article/10.1016/j.cj.2025.11.023},
doi = {10.1016/j.cj.2025.11.023},
abstract = {Late leaf spot (LLS) is a major foliar disease that significantly reduces yield and compromises the quality of peanut (Arachis hypogaea L.). To identify genes conferring LLS resistance, a recombinant inbred line (RIL) population of 257 lines from a cross between the susceptible cultivar Baisha 1016 and the resistant germplasm ICGV 86699 was analyzed using whole-genome resequencing. A high-density genetic map was constructed with 4908 bin markers, leading to the identification of 19 quantitative trait loci (QTL) for LLS resistance. A major QTL, qLLS.A02.1, was consistently detected on chromosome A02 across six environments, explaining up to 37.49% of the phenotypic variance. Fine mapping delimited the qLLS.A02.1 locus to a 319 kb region flanked by KASP markers S4 and S5, which contained a cluster of ten NBS-LRR genes. Validation in 266 accessions confirmed a significant association of these flanking markers with LLS resistance. Transcriptome analysis revealed that four NBS-LRR genes within the cluster were specifically upregulated in the resistant parent. This study provides valuable genetic resources and molecular markers for breeding LLS-resistant peanut varieties.}
}