TY  - JOUR
AU  - Li, Huadong
AU  - Luo, Lu
AU  - Yang, Hui
AU  - Li, Xing
AU  - Li, Yuying
AU  - Yu, Haiyang
AU  - Zhang, Kun
AU  - Dong, Yuanjie
AU  - Hou, Benjun
AU  - Miao, Penghui
AU  - Wan, Yongshan
AU  - Liu, Fengzhen
PY  - 2026
TI  - Fine mapping of the major QTL qLLS.A02.1 and identification of a novel NBS-LRR gene cluster associated with late leaf spot resistance in peanut (Arachis hypogaea L.)
JO  - The Crop Journal
SN  - 2095-5421
SP  - 547
EP  - 555
VL  - 14
IS  - 2
AB  - Late leaf spot (LLS) is a major foliar disease that significantly reduces yield and compromises the quality of peanut (Arachis hypogaea L.). To identify genes conferring LLS resistance, a recombinant inbred line (RIL) population of 257 lines from a cross between the susceptible cultivar Baisha 1016 and the resistant germplasm ICGV 86699 was analyzed using whole-genome resequencing. A high-density genetic map was constructed with 4908 bin markers, leading to the identification of 19 quantitative trait loci (QTL) for LLS resistance. A major QTL, qLLS.A02.1, was consistently detected on chromosome A02 across six environments, explaining up to 37.49% of the phenotypic variance. Fine mapping delimited the qLLS.A02.1 locus to a 319 kb region flanked by KASP markers S4 and S5, which contained a cluster of ten NBS-LRR genes. Validation in 266 accessions confirmed a significant association of these flanking markers with LLS resistance. Transcriptome analysis revealed that four NBS-LRR genes within the cluster were specifically upregulated in the resistant parent. This study provides valuable genetic resources and molecular markers for breeding LLS-resistant peanut varieties.
UR  - https://doi.org/10.1016/j.cj.2025.11.023
DO  - 10.1016/j.cj.2025.11.023