@article{DENG2025, 
author = {Wenhuan DENG and Fang HU and Zhiju XUE and Jinqi ZHOU and Yingxia ZHANG},
title = {Preparation of CMCS/HPMC/P407/P188 thermosensitive injectable composite hydrogel with Temporin-GHaR and its antibacterial effects},
year = {2025},
journal = {Natural Science of Hainan University},
volume = {43},
number = {6},
pages = {724-737},
keywords = {Temporin-GHaR, chitosan hydrogel, temperature sensitive material, drug sustained release, biocompatibility},
url = {https://www.sciopen.com/article/10.15886/j.cnki.hndk.2025042201},
doi = {10.15886/j.cnki.hndk.2025042201},
abstract = {In order to solve the problems of the poor stability and high hemolytic toxicity of Temporin-GHaR, the preparation design technology was used to construct a thermosensitive quaternary composite hydrogel of carboxymethyl chitosan/hydroxypropyl methylcellulose/poloxamer loaded with Temporin-GHaR (CHPP/GHaR). The response surface method was used to optimize the formulation, and the obtained hydrogel with a porous fiber network structure exhibited shear-thinning, self-healing, and injectability, with a swelling rate of 161.5%. The release experiments tests showed a sustained release of 55% within 40 hours with low burst release risk. Biocompatibility evaluation test confirmed that the hydrogel loaded with 200 μmol·L−1 GHaR had only 2.2% hemolysis rate and no significant cytotoxicity to keratinocytes and umbilical vein endothelial cells. In vitro antimicrobial experiments demonstrated that CHPP/GHaR achieved above 99% inhibition rates against methicillin-resistant Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Our findings provide a new candidate material for the treatment of the drug-resistant bacterial-infections.}
}