@article{ZHANG2026, 
author = {Xinyi ZHANG and Xiaoyu WU and Zihao TAO and Shuchang WEI and Lei ZHAO and Wenda DUAN and Yanlong PAN and Abuduaini Dilinigaer and Yinyun MA},
title = {Research advances in screening methods for pancreatic lipase inhibitors},
year = {2026},
journal = {Journal of China Pharmaceutical University},
volume = {57},
number = {2},
pages = {163-171},
keywords = {pancreatic lipase, inhibitor, p-nitrophenyl-based assay, fluorescence assay, immobilized enzyme method, affinity ultrafiltration with mass spectrometry, virtual screening},
url = {https://www.sciopen.com/article/10.11665/j.issn.1000-5048.2025092401},
doi = {10.11665/j.issn.1000-5048.2025092401},
abstract = {Obesity and its related metabolic diseases have become a major global public health threat, and its rising incidence significantly increases the risk of cardiovascular and cerebrovascular diseases, diabetes and other complications. Pancreatic lipase is a key enzyme that converts food-borne lipids into triglycerides and fatty acids, and the effective inhibition of its activity has become an important strategy for the treatment of obesity. This paper discusses the screening methods of pancreatic lipase inhibitors, and summarizes and reviews the basic principles, advantages and disadvantages and application status of traditional screening methods, modern new screening methods and virtual screening methods. In view of the problems faced by the screening methods of pancreatic lipase inhibitors, future research urgently needs to move towards a collaborative innovation path of multi-technology integration, intelligent screening and complex systematization of traditional Chinese medicine, so as to open up new research paradigms.}
}