@article{Guo2026, 
author = {Zhenying Guo and Chunwei Xu and Shirong Zhang and Yue Hao and Xiaotong Hu and Ming Zhao and Chan Xiang and Yingshi Piao and Pingli Sun and Xueping Xiang and Jing Zhao and Huanwen Wu and Weixing Li and Jinpu Yu and Jingping Yuan and Shuangshuang Wang and Cong Wang and Yun Gu and Bingjian Lv and Liping Zhang and Yueping Liu and Xiaobin Cui and Weizhong Gu and Yining Li and Wei Wang and Wenjun Yang and Weiguo Long and Jingjing Xiang and Hong Mou and Biao Liu and Huajuan Ruan and Yubin Wang and Yongjie Zhu and Feng Wang and Zhonghua Wang and Xiaomin Feng and Xing Liu and Peng Li and Min Deng and Bin Lian and Lili Mao and Qian Wang and Wenxian Wang and Zhengbo Song and Ziming Li and Wenzhao Zhong and Zhijie Wang and Shengxiang Ren and Wenfeng Fang and Yongchang Zhang and Jingjing Liu and Xiuyu Cai and Anwen Liu and Wen Li and Ping Zhan and Hongbing Liu and Tangfeng Lv and Liyun Miao and Lingfeng Min and Yu Chen and Yu Zhang and Feng Wang and Zhansheng Jiang and Gen Lin and Long Huang and Xingxiang Pu and Rongbo Lin and Weifeng Liu and Chuangzhou Rao and Dongqing Lv and Zongyang Yu and Peng Shen and Xiaoyan Li and Chuanhao Tang and Chengzhi Zhou and Junping Zhang and Junli Xue and Hui Guo and Qian Chu and Rui Meng and Jingxun Wu and Rui Zhang and Jin Zhou and Zhengfei Zhu and Yongheng Li and Hong Qiu and Fan Xia and Yuanyuan Lu and Xiaofeng Chen and Rui Ge and Enyong Dai and Yu Han and Jian Zhang and Yinghua Ji and Xianbin Liang and Hongmei Zhang and Xuelei Ma and Xuewen Liu and Yu Yao and Peng Luo and Weiwei Pan and Fei Pang and Fan Wu and Dejian Gu and Li Wang and Liping Wang and Youcai Zhu and Li Lin and Weiwen Li and Xinqing Lin and Jing Cai and Ling Xu and Jisheng Li and Xiaodong Jiao and Kainan Li and Jia Wei and Huijing Feng and Lin Wang and Yingying Du and Wang Yao and Xuefei Shi and Xiaomin Niu and Dongmei Yuan and Yanwen Yao and Yinbin Zhang and Binbin Song and Wenfeng Li and Jianfei Fu and Hong Wang and Mingxiang Ye and Dong Wang and Zhaofeng Wang and Qing Ji and Yuan Fang and Qing Wei and Zhen Wang and Bin Wan and Donglai Lv and Xiaofeng Li and Shengjie Yang and Jing Kang and Jiatao Zhang and Chao Zhang and Lin Shi and Yina Wang and Bihui Li and Zhang Zhang and Ke Wang and Zhefeng Liu and Nong Yang and Lin Wu and Xiaobing Chen and Gu Jin and Zhongwu Li and Miao Li and Guansong Wang and Jiandong Wang and Meiyu Fang and Yong Fang and Xiaojia Wang and Jing Chen and Yiping Zhang and Xixu Zhu and Yi Shen and Shenglin Ma and Biyun Wang and Lu Si and Yong Song and Yuanzhi Lu and Aijun Liu and Yuchen Han},
title = {Expert consensus on the detection and clinical application of tumor mutational burden},
year = {2026},
journal = {Cancer Biology & Medicine},
volume = {23},
number = {2},
pages = {218-246},
keywords = {Biomarkers, tumor mutational burden, tumor immunotherapy, targeted panel sequencing, whole-exome sequencing},
url = {https://www.sciopen.com/article/10.20892/j.issn.2095-3941.2025.0351},
doi = {10.20892/j.issn.2095-3941.2025.0351},
abstract = {As an emerging biomarker, tumor mutational burden (TMB) has attracted increasing attention from clinicians in predicting the efficacy of tumor immunotherapy. Currently, TMB is detected primarily by whole-exome sequencing or targeted panel sequencing on high-throughput sequencing platforms. However, the lack of uniformity in detection methods, threshold settings, and reporting formats, as well as the significant differences in TMB values among different cancer types, have hindered the standardized application of this biomarker in clinical practice. This consensus focuses on the definition, standardization of detection, clinical significance, and limitations of TMB, and provides consensus recommendations for the clinical application of TMB in real-world practice in China. This consensus is aimed at helping clinicians and laboratory personnel understand the clinical significance and testing standards of TMB, promoting more accurate interpretation of test results, and improving patient care.}
}