@article{Han2026, 
author = {Jincheng Han and Wenhan Kang and Yang Wan and Xiwen Miao and Jiukai Zhang and Ning Yu and Ying Chen},
title = {Allergy Off Switch: Targeted Immune Tolerance Peptide for Alpha-Lactalbumin Allergy},
year = {2026},
journal = {Food Science and Human Wellness},
keywords = {Functional food, Oral tolerance, Prevention of cow's milk allergy, Peptide-based immunotherapy, Mouse model of cow's milk allergy, dietary therapy},
url = {https://www.sciopen.com/article/10.26599/FSHW.2026.9251030},
doi = {10.26599/FSHW.2026.9251030},
abstract = {Alpha-lactalbumin (ALA), a significant allergen in cow's milk, requires urgent preventative and therapeutic techniques. Peptide immunotherapy may modify allergic reactivity without cross-linking IgE, making it crucial to milk allergy treatment. In the present study, tolerogenic peptides of α-lactalbumin have been identified by constructing immune cells (MLN cells, splenocytes) from a mouse model of cow's milk allergy and co-culturing them in vitro with candidate peptides. The allergy model demonstrated significant alterations in physiological and biochemical indices within the experimental group. Subsequent co-culture of immune cells with candidate peptides revealed that peptide ALA AA (1-19) induced the expansion of Treg cells and promoted a tolerogenic phenotype in DCs. Taken together, these results led to the successful screening of ALA-tolerant peptides, which inhibited the release of allergy-related cytokines and induced the differentiation of immune cells toward the tolerant phenotype. This study provides a food-compatible peptide strategy for CMA management, with potential applications in hypoallergenic dairy products. Meanwhile, these findings laid the foundation for further validation in subsequent in vivo experiments.}
}