@article{LI2026, 
author = {Xingyan LI and Wenjie REN and Yulong WANG and Zhihua WANG and Yuxiu HAN and Jiani LI and Kaichan REN and Baoshan HE},
title = {Rapid Detection of Ochratoxin A in Corn Using a Digoxin-Modified Aptamer-Based Lateral Flow Test Strip},
year = {2026},
journal = {Food Science},
volume = {47},
number = {1},
pages = {8-16},
keywords = {aptamer, digoxin, chromatographic test strip, ochratoxin A},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20250829-210},
doi = {10.7506/spkx1002-6630-20250829-210},
abstract = {To establish a rapid and sensitive method for detecting ochratoxin A (OTA), a lateral flow test strip was developed using a gold nanoparticle-c DNA conjugate (Au NPs-SH-c DNA) by a freezing method as the detector probe on the conjugate pad as well as using anti-digoxin monoclonal antibodies as the test line and aptamers as the control line on the nitrocellulose membrane. Digoxin-modified aptamers (Dig-Apt) were used as recognition elements for binding to OTA in the loading buffer. Under optimal conditions, the limit of detection (LOD) of the method was 0.3 ng/mL, and the half-maximal inhibitory concentration (IC50) was 12.5 ng/mL. The recoveries in spiked corn samples ranged from 100.82% to 106.30%, with coefficients of variation between 2.54% and 6.78%, a false positive rate ≤ 0% and a false negative rate ≤ 1%. The developed test strip provides an effective method for detecting OTA in corn, demonstrating promising application potential.}
}