TY  - JOUR
AU  - WANG, Maolin
AU  - LIU, Yan
AU  - YAN, Fang
AU  - SUN, Guibin
PY  - 2025
TI  - Progress of cross-linking mass spectrometry based on liquid chromatography-mass spectrometry technology in the research of protein-protein interactions
JO  - Journal of China Pharmaceutical University
SN  - 1000-5048
SP  - 793
EP  - 802
VL  - 56
IS  - 6
AB  - Cross-linking mass spectrometry (XL-MS) technology has become a key tool for identifying protein-protein interactions (PPIs) and revealing the protein structures in living cells, tissues, or organelles. The XL-MS technology has demonstrated remarkable superiority compared with conventional methods for studying PPIs and protein structures. In the past few years, new cross-linker structures have been arising continuously. They have not only broadened the range of cross-linking reactions but also provided additional methods for the enrichment of cross-linking peptides. Moreover, owing to the advancement of computer software and artificial intelligence, cross - linking data search engines and visualization software have also been enhancing the speed and accuracy of cross-linking peptide identification. The evolution of cross-linkers and cross-linking peptide search engines has promoted the application of the XL-MS technology in the research on protein-protein interactions (PPIs) within organelles and even living cells and in the analysis of protein structures.
UR  - https://doi.org/10.11665/j.issn.1000-5048.2025021401
DO  - 10.11665/j.issn.1000-5048.2025021401