@article{ZHU2025, 
author = {Feng1 ZHU and Chaoting FU and Yazhou WANG and Zheng KUANG and Lifang YIN},
title = {Redox-responsive nanoparticles reversing non-small cell lung cancer multidrug resistance via dual mechanisms},
year = {2025},
journal = {Journal of China Pharmaceutical University},
volume = {56},
number = {6},
pages = {729-736},
keywords = {redox-responsive nanoparticles, multidrug resistance, paclitaxel, non-small cell lung cancer},
url = {https://www.sciopen.com/article/10.11665/j.issn.1000-5048.2025060701},
doi = {10.11665/j.issn.1000-5048.2025060701},
abstract = {A redox-responsive hyaluronic acid-vitamin E polyethylene glycol succinate nanoparticle loaded with paclitaxel (HA-SS-TPGS@PTX) was designed to investigate its mechanism for overcoming multidrug resistance (MDR) in non-small cell lung cancer (NSCLC) in vitro. HA-SS-TPGS@PTX nanoparticles were prepared using an emulsion-ultrasonication method. Techniques such as flow cytometry and confocal laser scanning microscopy (CLSM) were employed to study their effects on apoptosis induction, mitochondrial function, and the regulation of P-glycoprotein (P-gp) expression in PTX-resistant lung cancer cells (A549/T). Results showed that HA-SS-TPGS@PTX nanoparticles significantly inhibited the proliferation of A549/T cells in vitro, with an IC50 of 1.35 μg/mL. The nanoparticles entered the cells via CD44 receptor-mediated endocytosis. The high intracellular concentration of glutathione (GSH) triggered the release of PTX and TPGS, which subsequently induced a decrease in mitochondrial membrane potential, leading to apoptosis. Meanwhile, HA-SS-TPGS@PTX also inhibited P-gp expression and ATP consumption, thereby blocking drug efflux. The design of HA-SS-TPGS@PTX provides a new strategy for overcoming MDR in NSCLC.}
}