@article{LI2025, 
author = {Qin LI and Yupeng WEN and Wendi HUO and Huan LIU and Yingchun MU and Jincheng LI},
title = {Fast and simultaneous determination of ten prohibited and restricted tranquilizers in fish samples by HPLC-MS/MS},
year = {2025},
journal = {Journal of Capital Normal University (Natural Science Edition)},
volume = {46},
number = {6},
pages = {1-8},
keywords = {aquatic products, tranquilizer determination, high performance liquid chromatographytandem mass spectrometry},
url = {https://www.sciopen.com/article/10.19789/j.1004-9398.2025.06.001},
doi = {10.19789/j.1004-9398.2025.06.001},
abstract = {In order to quickly determine three types of prohibited and restricted sedatives in fish, a rapid high performance liquid chromatography-tandem mass spectrometry method was explored for the simultaneous determination of three types of cocaine, one type of non-barbiturates, and six types of benzodiazepine tranquilizers in fish samples. The sample was extracted with acetonitrile, rapidly purified by low-temperature high-speed centrifugation, fast separated by ultra-performance liquid chromatography, and detected by online-coupled triple quadrupole/composite linear ion trap mass spectrometer. The linear working curves were measured in the concentration range of 0.2 to 20.0 μg/L, with linear correlation coefficients all higher than 0.999 0, and quantification limits at 1.0 μg/kg in fish. The recovery was measured by spiking 10 tranquilizer standards into carp muscle tissue at three levels of 1.0, 2.0 and 5.0 μg/kg, giving averaged data all within the range between 86.5% and 118.5%, with relative standard deviations between 2.2% and 7.7%. The method is validated to be simple, rapid, efficient, sensitive and accurate, being usable as a routine method for the determination of 10 types of tranquilizers in aquatic products.}
}