@article{Lü2025, 
author = {Xinran Lü and Yangrui Wang and Dongyu Chang and Fengling Bai and Xin Wen and Mengmin Lü and Xueqing Liu and Jianrong Li and Xuepeng Li and Defu Zhang and Yuqiong Meng and Rui Ma},
title = {Inhibition mechanism of quorum sensing and biofilm formation of Pseudomonas fluorescens by a novel quorum quenching bacteria Lactiplantibacillus plantarum YP4-1-2},
year = {2025},
journal = {Food Science and Human Wellness},
volume = {14},
number = {12},
pages = {9250294},
keywords = {Quorum sensing, Quorum quenching, Pseudomonas fluorescens, Lactiplantibacillus plantarum YP4-1-2, Penicillin V acylase},
url = {https://www.sciopen.com/article/10.26599/FSHW.2024.9250294},
doi = {10.26599/FSHW.2024.9250294},
abstract = {Pseudomonas fluorescens is a widespread spoilage bacterium in food, and its spoilage characteristics and biofilm formation are regulated by N-acyl-homoserine lactone (AHLs) quorum sensing (QS) system. Quorum quenching (QQ) is considered as an effective strategy to control the spoilage bacteria. Therefore, this study revealed a new QQ bacteria Lactiplantibacillus plantarum YP4-1-2. Notably, the crude cell extract (CCE) from L. plantarum YP4-1-2 showed strongly QQ activity against the AHLs of P. fluorescens. The degradation rate of AHLs (N-octanoyl-L-homoserine lactone) reached 25.90%, 78.57% and 100% at the CCE protein concentrations of 57, 86 and 114 μg/mL, respectively. In addition, the CCE could drastically reduce the formation of biofilm, bacterial motility (swimming and swarming), and the release of extracellular protease and biogenic amines of P. fluorescens. Real-time polymerase chain reaction (PCR) results revealed that the CCE downregulated QS-related genes (rhlI, rhlR, aprX, algA, orm, flgA, and ldcA). Finally, using whole-genome sequencing analysis, the active substance in the CCE was identified to be penicillin V acylase (PVA) and named LpPVA. Meanwhile, homologous modeling and molecular docking analysis showed that the binding effect of LpPVA on long-chain AHLs was better than that on short-chain AHLs. This study demonstrated that L. plantarum YP4-1-2 could be considered as a promising QS inhibitor and antibiofilm agent against foodborne spoilage bacteria.}
}