@article{Hao2026, 
author = {Zhilong Hao and Yuping Zhang and Weiyi Kong and Jiao Feng and Yucheng Zheng and Hongzheng Lin and Xiaomin Yu and Yun Sun and Xiangxiang Huang and Wei Wang and Yang Wu and Xinyi Jin},
title = {Mechanical stress induces molecular changes in oolong tea: Insights from multi-omics analysis},
year = {2026},
journal = {Journal of Integrative Agriculture (JIA)},
volume = {25},
number = {1},
pages = {352-365},
keywords = {oolong tea, multi-omics, mechanical stress, defense response, α-linolenic acid metabolism, phenylalanine metabolism},
url = {https://www.sciopen.com/article/10.1016/j.jia.2025.09.014},
doi = {10.1016/j.jia.2025.09.014},
abstract = {Understanding the molecular responses of tea leaves to mechanical stress is crucial for elucidating the mechanisms of post-harvest quality formation during oolong tea processing. This study employed an integrated multi-omics strategy to characterize the changes and interactions among metabolomic (MB), transcriptomic (TX), and proteomic (PT) profiles in mechanically stressed tea leaves. Mechanical stress initially activated damage-associated molecular patterns (DAMPs), including Ca2+ signaling, jasmonic acid signaling, and glutathione metabolism pathways. These processes subsequently induced quality-related metabolic pathways (QRMPs), particularly α-linolenic acid and phenylalanine metabolism. Up-regulated expression of LOX, ADH1, and PAR genes, together with the increased abundance of their encoded proteins, respectively promoted the accumulation of jasmine lactone, benzyl alcohol, and 2-phenylethanol. These findings indicate that mechanical stress influences the metabolite biosynthesis in tea leaves through coordinated molecular responses. This study provides new insights into the molecular mechanisms underlying tea leaf responses to mechanical stress and a foundation for future investigations into how early molecular events may contribute to post-harvest metabolic changes during oolong tea processing.}
}