@article{Zhang2025, 
author = {Xiaobo Zhang and Xiuwen Wang and Longjiao Zhu and Jinyan Zhu and Bing Hu and Qiuyue Zheng and Jingli Yuan and Wentao Xu and Jijuan Cao},
title = {Nanoconfinement boosts electrochemiluminescence based on in-situ anchoring Pt NCs on mesoporous Mn3O4 for rapid and sensitive detection of salmonella typhimurium},
year = {2025},
journal = {Food Science and Human Wellness},
keywords = {electrochemiluminescence aptasensor, Salmonella typhimurium, nanoconfinement, mesoporous structure, Pt nanoclusters},
url = {https://www.sciopen.com/article/10.26599/FSHW.2025.9250845},
doi = {10.26599/FSHW.2025.9250845},
abstract = {The development of a simple, rapid and sensitive method for foodborne pathogens is essential to ensure public health safety. Existing methods, such as culture-based techniques and polymerase chain reaction (PCR), often face challenges in terms of time-consuming, labor-intensive, and require extensive sample preparation and amplification, which makes them impractical for rapid or on-site pathogen detection. Herein, a competitive mechanism-driven electrochemiluminescence (ECL) aptasensor was constructed for Salmonella typhimurium (S. typhimurium) detection without nucleic acid extraction and amplification. To boost the ECL signal of luminol-H2O2 systems, in-situ anchoring of Pt nanoclusters on mesoporous Mn3O4 nanoparticles (Pt NCs@M-Mn3O4) used as the nanoconfinement co-reactor. Benefitting from the restricted mesoporous structure, Pt NCs@M-Mn3O4 provided a unique microenvironment for the enrichment of H2O2. Furthermore, the interfacial interaction could activate the electronic transfer between active species of Pt NCs and M-Mn3O4, enabling a synergistic catalytic effect on ROS generation to effectively boost the ECL emission of luminol. The proposed ECL aptasensor had a linear concentration from 2 × 101 to 2 × 106 CFU/mL with limit of detection of 8 CFU/mL of S. typhimurium. Furthermore, the method showed high anti-interference ability. Especially, satisfactory recovery values in spiked-actual samples were obtained, thereby demonstrating great potential for sensitive and reliable detection of S. typhimurium in various environments.}
}