@article{ZHA2025, 
author = {Ao ZHA and Liwen JIANG and Xiaochen LIU and Yang LIU and Xinmeng LIU and Chen LI and Shulong CAI and Liqi FAN and Jucai XU},
title = {Analysis of sequence composition characteristics and peptide mining of soy protein isolate},
year = {2025},
journal = {China Brewing},
volume = {44},
number = {8},
pages = {114-119},
keywords = {soy protein isolate, proteomics, peptidomics, bioactive peptides, umami peptides},
url = {https://www.sciopen.com/article/10.11882/j.issn.0254-5071.2025.08.018},
doi = {10.11882/j.issn.0254-5071.2025.08.018},
abstract = {In this study, the sequence composition of soybean protein isolate (SPI) was identified by proteomics combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The bioactive and umami peptides were mined by high-abundance peptide sequences in SPI based on peptitomics analysis, and in combination with the peptide database and prediction model. The results indicated that SPI was mainly composed of 8 protein sequences, among which the sequences P11827, P0DO16, and P25974 corresponding to the α, α', and β subunits in β-conglycinin, respectively, accounting for 42.49%. The sequences P04776, P04005, P11828, P04347, and P02858 were derived from the 5 subunits (G1 to G5) in glycinin, accounting for 57.51%. It was statistically found that the relative contents of dipeptides such as glutamine-glutamic acid (EE), glutamine-glutamyl (QQ), and aspartic amine-glutamic acid (DE) in the SPI sequence were relatively high, which were 3.74%, 3.07%, and 2.84%, respectively. The repeat features of dipeptide starting and ending with E or Q in the corresponding sequence were prominent, with theoretical maximum contents all greater than 16%, and they had high potential umami characteristics or hypoglycemic activities, fully demonstrating the wide application prospects of SPI in functional umami agents.}
}