@article{TAO2025, 
author = {Wei TAO and Haibing LIU and Mingyu GUO and Yongyuan LIU and Elvis Kwame ADINKRA and Yuqi LI and Yuexin MA and Yu CHEN and Chuanchao WU and Yan LIU},
title = {Effect of Surfactant-Mediated Membrane Permeabilization on Menaquinone-7 Production by Engineered Bacillus subtilis},
year = {2025},
journal = {Food Science},
volume = {46},
number = {19},
pages = {98-106},
keywords = {Bacillus subtilis, surfactant, cell membrane, permeabilization, menaquinone-7, transcriptomics},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20250402-012},
doi = {10.7506/spkx1002-6630-20250402-012},
abstract = {In this study, surfactant-induced mediated cell membrane permeabilization was explored as a strategy to increase the production of menaquinone-7 (MK-7) by an engineered strain, BS168-ΔSinR. Various surfactants were screened for their effectiveness in improving the MK-7 production of BS168-ΔSinR. Cell morphology was observed by scanning electron microscopy (SEM), and cell membrane permeability was detected by flow cytometry and fluorescence microscopy. RNA-seq was used to analyze the expression levels of genes related to MK-7 production. The results showed that the addition of 0.7% Brij-58 significantly increased the yield of MK-7, resulting in a 71.95% and 332.29% increase in the yield of total and extracellular MK-7 when compared with the control strain, respectively. The results from SEM, flow cytometry, and fluorescence microscopy demonstrate that Brij-58-mediated membrane permeabilization was significantly associated with enhanced MK-7 production. RNA-seq analysis showed that the expression levels of genes involved in MK-7 biosynthesis and the antioxidant defense system were generally up-regulated, while the expression levels of genes involved in spore formation were down-regulated. The results of this study provide a theoretical basis for the industrial production of MK-7, and reveal the mechanism by which surfactant-mediated membrane permeabilization increases the yield of MK-7.}
}