@article{LAN2025, 
author = {Zishu LAN and Shuqing ZHOU and Tianjiao CHEN and Rong TAN and Min JIN and Dong YANG},
title = {RPA-CRISPR/Cas12a-based rapid detection of Clostridium botulinum in food},
year = {2025},
journal = {Military Medical Sciences},
volume = {49},
number = {9},
pages = {674-680},
keywords = {food, Clostridium botulinum, CRISPR-Cas12a, rapid detection},
url = {https://www.sciopen.com/article/10.7644/j.issn.1674-9960.2025.09.005},
doi = {10.7644/j.issn.1674-9960.2025.09.005},
abstract = {ObjectiveTo establish a rapid detection method for Clostridium botulinum in food.MethodsA rapid detection method based on RPA-CRISPR/Cas12a was developed by integrating recombinase polymerase amplification (RPA) with the CRISPR-Cas12a system. After the reaction conditions were optimized, the method’s sensitivity, specificity, and usefulness were methodically confirmed.Results and ConclusionThe optimized method achieved detection within 1 hour, with a limit of detection (LOD) of 1.91 copies/μL. No cross-reactivity was observed with non-target pathogens. The RPA-CRISPR/Cas12a-based detection method developed in this study exhibits high specificity, sensitivity, and operational simplicity and may provide a feasible solution for the rapid detection of foodborne pathogens.}
}