@article{OUYANG2016, 
author = {Ke-xiong OUYANG and Jun LIANG and Rui ZOU and Zhi-qiang LI and Zhi-bao BAI and Zheng-guo PIAO and Jian-Jiang ZHAO},
title = {Ion Torrent RNA-Seq detection and analysis of the long non-coding RNA in tongue squamous cell carcinoma},
year = {2016},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {24},
number = {1},
pages = {15-19},
keywords = {Long non-coding RNA, Tongue squamous cell carcinoma, High-throughput sequencing},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2016.01.003},
doi = {10.12016/j.issn.2096-1456.2016.01.003},
abstract = {ObjectiveTo study the difference of LncRNA expression profiles between tongue squamous cell carcinoma (TSCC) and the corresponding adjacent normal tissue. To explore the role of long non-coding RNA in gene regulation.MethodsOne fresh sample of TSCC tissue and the adjacent normal tongue tissue were selected. After extracting the total RNA, the LncRNA from different tissues was screened and compared by Ion Torrent RNA-Seq. Pick LncRNA read sequence by the method used Bowtie2, then calculate the reads per kilo bases per million reads, RPKM of LncRNA by the method then Cufflinks. Choose the RPKM ratio of LncRNA from Cancer and adjacent tissue as a candidate target.ResultsAccording to RNA-Seq, 52 LncRNA whose value of RPKM with a fold ＞ 10 or ＜ 0.1 compared to the adjacent normal tongue tissue was enrolled in this study, of which 28 was up-regulated expression, 24 was down-regulated expression.ConclusionLncRNA expression profiles may be an important potential targets in TSCC.}
}