@article{YANG2025, 
author = {Zhe YANG and Min DUAN and Yumeng YE and Yongyi WANG and Jiao ZHANG and Xuejia WANG and Jun WANG and Yang LI},
title = {Effects of myeloid cell-specific knockout of G-CSFR on the progression of acute radiation pneumonitis in mice},
year = {2025},
journal = {Military Medical Sciences},
volume = {49},
number = {8},
pages = {582-588},
keywords = {ionizing radiation, mice, radiation-induced pneumonia, granulocyte colony-stimulating factor, neutrophils},
url = {https://www.sciopen.com/article/10.7644/j.issn.1674-9960.2025.08.004},
doi = {10.7644/j.issn.1674-9960.2025.08.004},
abstract = {ObjectiveTo investigate the impact of myeloid cell-specific knockout of the granulocyte colony-stimulating factor receptor (G-CSFR) on the progression of acute radiation pneumonitis.MethodsMyeloid cell-specific G-CSFR knockout (G-CSFR-/-, Lyz2-cre) mice were constructed. G-CSFR-/-, Lyz2-cre and C57BL/6N mice underwent a single whole-body irradiation with 6.5 Gy of 60Co γ-rays to establish a model of radiation injury. The lung function of mice was assessed using a mouse lung function test system at 3, 7 and 14-days post γ-ray irradiation. Pathological changes in the lung tissue were analyzed via hematoxylin and eosin (HE) staining of paraffin sections. Tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) levels were measured via radioimmunoassay. IL-8 and its receptor CXCR2 were quantified using enzyme-linked immunosorbent assay (ELISA). The infiltration of neutrophils in lung tissue was evaluated by immunohistochemical detection of myeloperoxidase.ResultsAt 3-, 7- and 14-days post-irradiation with 6.5 Gy of 60Co γ-rays, there were no significant differences observed in lung function or interstitial inflammatory lesions between G-CSFR-/-, Lyz2-cre mice and C57BL/6N mice. However, the infiltration of neutrophils in lung tissue of G-CSFR-/-, Lyz2-cre mice was significantly reduced (P＜0.01), and the levels of IL-8, CXCR2 and TNF-α in lung tissues were markedly lower than in C57BL/6N mice (P＜0.05).ConclusionThe myeloid cell-specific knockout of G-CSFR can effectively diminish neutrophil infiltration as well as inflammatory cytokine levels in lung tissues following radiation exposure.}
}