@article{ZHOU2023, 
author = {Qingnan ZHOU and Huanying ZHAO and Shuang CAI and Ningyan YANG and Yan ZHOU and Jiajian SHANG},
title = {A short-term analysis of the modification process of the plaque microbial community before and after treatment in children with S-ECC},
year = {2023},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {31},
number = {4},
pages = {267-273},
keywords = {severe early childhood caries, dental general anesthesia, oral microecology, plaque microbial community, microbiome diversity, dysbiosis, biofilm, caries management},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2023.04.007},
doi = {10.12016/j.issn.2096-1456.2023.04.007},
abstract = {ObjectiveTo explore from the perspective of microorganisms the changes in plaque microbial community of children with severe early childhood caries (S-ECC) before and 3 months after dental treatment. Meanwhile to show the effect of treatment on the maintenance of long- term caries-free state.MethodsS-ECC children completed dental treatment under general anesthesia. We collected plaque from caries-free dental surfaces before treatment (caries, C) and at the postoperative follow-up review time points of 7 days (C-7D), 1 month (C-1 M), and 3 months (C-3 M). We included caries-free children (caries free, CF) as the control group to analyze the dynamic modification process of the plaque microbial community in the short-term pre- and postdental treatment.ResultsSpecies clustering analysis showed that the compositions of the microbial communities of the S-ECC and CF groups were highly similar. The α diversity index was not statistically significant (P＞0.05). From the analysis of the relative abundance, Leptotrichia spp. and Aggregatibacter spp. decreased after treatment compared with before treatment (P＜0.05). Streptococcus sanguinis in the C-7D group increased compared with that in the C group and gradually decreased within 3 months. Veillonella spp., Actinomyces spp., Allprevotella spp., Capnocytophaga spp., and Streptococcus mutans differed between the C and CF groups (P＜0.05), Streptococcus mutans did not differ significantly between the C-7D and C-1 M groups and the CF group after treatment, while C-3 M showed an increase compared with the CF group (P＜0.01).ConclusionThe rapid change in the structure of the flora of children with S-ECC after treatment. The plaque microbial community structure in a caries-free state gradually starts to be established 1-3 months after treatment. There is a "core microbiota" in the oral plaque community that jointly maintains microecological stability. Veillonella spp., Allprevotella spp. and Streptococcus mutans have potential as possible microbial markers.}
}