@article{DENG2022, 
author = {Zilong DENG and Wenjuan YAN and Wanghong ZHAO and Buling WU},
title = {Effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells},
year = {2022},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {30},
number = {11},
pages = {779-784},
keywords = {dental pulp stem cells, proliferation, migration, N-cadherin, dental pulp regeneration, cell cycle, cell apoptosis, lentivirus, microenvironment},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2022.11.003},
doi = {10.12016/j.issn.2096-1456.2022.11.003},
abstract = {ObjectiveTo investigate the effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells (DPSCs) and to provide experimental evidence for DPSCs-based dental pulp regeneration.MethodsDPSCs were transfected with N-cadherin shRNA lentivirus, and the knockdown efficiency of N-cadherin at both the mRNA and protein levels was confirmed by qRT-PCR and Western blot. The experiment included a negative control group (shRNA -NC) and an N-cadherin shRNA silencing group. Cell proliferation was detected by the CCK-8 method. Cell cycle and apoptosis were assessed by flow cytometry, and cell migration was detected using the Transwell method.ResultsN-cadherin shRNA significantly reduced the expression levels of N-cadherin mRNA and protein in DPSCs (P<0.001). The proliferation activity of the N-cadherin shRNA group was significantly greater than that of the shRNA-NC group on the 3rd and 4th days after cell inoculation and lower than that of the shRNA-NC group from the 6th to 8th days (P<0.05). On the 3rd day after cell inoculation, the proportion of cells in S phase and G2 phase in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.05). On the 6th day after cell inoculation, the proportion of cells in S phase and G2 phase in the N-cadherin shRNA group was lower than that in the shRNA-NC group (P<0.05), and the proportion of apoptotic cells in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.01). Low densities cells and high densities cells were inoculated into Transwell upper chamber for 20 h, the number of cells passing through the membrane pores of upper chamber in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.001).ConclusionSilencing N-cadherin expression can promote the early proliferation and migration of DPSCs.}
}