@article{ZHONG2021, 
author = {qijian ZHONG and Tingting JIN and Yu PENG and Weixiong CHEN and Jinsong LI},
title = {The effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells},
year = {2021},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {29},
number = {8},
pages = {535-540},
keywords = {endoplasmic reticulum stress, calnexin, tongue squamous cell carcinoma, SCC-9 cells, SCC-25 cells, proliferation, invasion, migration, siRNA, therapeutic target},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2021.08.005},
doi = {10.12016/j.issn.2096-1456.2021.08.005},
abstract = {Objective   To investigate the effect of silencing the endoplasmic reticulum stress-related protein calnexin on the proliferation, invasion, and migration of tongue squamous cell carcinoma cells. Methods   Calnexin siRNA was transfected into SCC-9 and SCC-25 tongue squamous cell carcinoma cells, and the expression of calnexin was detected by qRT-PCR. The silencing effect of calnexin siRNA was further verified by Western blotting. CCK-8 assay was applied to detect the effect of silencing calnexin on the proliferation of tongue squamous cell carcinoma cells; Transwell assay was used to detect the effect of silencing calnexin on the invasion and migration of tongue squamous cell carcinoma cells. Results   qRT-PCR showed that calnexin siRNA could effectively downregulate the expression of calnexin. Western blot analysis further confirmed the silencing effect of calnexin siRNA on calnexin. The CCK-8 assay showed that silencing calnexin expression on the 4th and 5th days could inhibit the proliferation of tongue squamous cell carcinoma cells, and the difference was statistically significant (P < 0.01). The Transwell assay showed that knockdown of calnexin could inhibit the invasion and migration of tongue squamous cell carcinoma cells (P < 0.001). Conclusion   Knockdown of calnexin can inhibit the proliferation, invasion, and migration of tongue squamous cell carcinoma cells.}
}