@article{QI2021, 
author = {Xia QI and Lingxue KONG and Shujuan LI and Siting MA and Yali QI and Lei ZHAO},
title = {Inhibitory effects of epigallocatechin-3-gallate on the pathogenic properties of P. gingivalis in vitro},
year = {2021},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {29},
number = {5},
pages = {314-321},
keywords = {epigallocatechin-3-gallate, Porphyromonas gingivalis, biofilm formation, biofilm reduction, arginine gingipain, lysine gingipain, antibacterial, anti-inflammatory, gingival fibroblasts, matrix metalloproteinase},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2021.05.004},
doi = {10.12016/j.issn.2096-1456.2021.05.004},
abstract = {ObjectiveTo explore the antibacterial activity of epigallocatechin-3-gallate (EGCG) on P. gingivalis and the inhibitory effects on matrix metalloproteinases (MMPs) production induced by P. gingivalis.MethodsThe antimicrobial effect of EGCG against planktonic cultures and biofilms of P. gingivalis was evaluated using microplate dilution assays. The microstructural changes in biofilms were studied using scanning electron microscopy (SEM). The inhibitory effect of EGCG on arginine gingipain (Rgp) and lysine gingipain (Kgp) activity of P. gingivalis was evaluated using synthetic chromogenic peptides and fluorogenic substrates. Enzyme-linked immunosorbent assay (ELISA) and qRT-PCR analysis were used to assess MMP-1 and MMP-2 mRNA expression and secretion by human gingival fibroblasts (HGFs) stimulated with P. gingivalis in the presence or absence of EGCG, respectively.ResultsThe MIC and MBC of EGCG against P. gingivalis were 62.5 μg/mL and 500 μg/mL, respectively. EGCG can not only inhibit the biofilm formation of P. gingivalis but also has a scavenging effect on mature biofilms and can affect their viability. Additionally, 10 μg/mL and 50 μg/mL of EGCG inhibited the proteinase activities of Rgp and Kgp, respectively (P < 0.05). Finally, the mRNA expression and secretion of MMP-1 and MMP-2 by HGFs stimulated by P. gingivalis were significantly inhibited by 50 μg/mL of EGCG (P < 0.05).ConclusionEGCG exhibits antimicrobial effects against P. gingivalis and reduces the expression of MMPs by HGFs.}
}