@article{REN2019, 
author = {Qingyuan REN and Wulin HE and Qing WANG and Hongxing CHU and Haiyan LIN},
title = {Effect of endoplasmic reticulum stress on the osteogenic differentiation of periodontal ligament cells under continuous static pressure},
year = {2019},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {27},
number = {8},
pages = {485-489},
keywords = {human periodontal ligament cells, PERK - eIF2α - ATF4 pathway, endoplasmic reticulum stress, continuously compressive pressure, osteogenic differentiation},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2019.08.002},
doi = {10.12016/j.issn.2096-1456.2019.08.002},
abstract = {ObjectiveTo study the effect of continuous static pressure on the endoplasmic reticulum of human periodontal ligament cells (hPDLCs) and the mechanism of osteogenic differentiation.MethodshPDLCs cultured in vitro were subjected to 1 g/cm2 of continuous compressive pressure (CCP) by custom-made, round, glass panes for 0, 2, 4, and 6 h, respectively. Alkaline phosphatase staining was used to detect osteogenic differentiation, and real-time quantitative PCR was used to detect the expression of protein kinase receptor-like ER kinase (PERK), eukaryotic translation initiation factor 2α (eIF2α), and transcription activation factor 4 (ATF-4). The 0 h loading group was the control group. ResultsAfter CCP treatment, the alkaline phosphatase staining of hPDLCs was blue-violet and significantly stronger than that of cells in the control group. The expression levels of PERK and ATF4 in the hPDLCs after CCP treatment were higher than those of cells in the control group (P < 0.05) and increased over time (P < 0.05). The expression of eIF2α was lower in the experimental groups than in the control group (P < 0.05) and decreased over time (P < 0.05).ConclusionMechanical stimulation can activate ERS in hPDLCs, leading to enhanced PERK-eIF2α-ATF4 signaling and inducing osteogenic differentiation.}
}