@article{XU2018, 
author = {Jing XU and Jimin XIONG and Pengju XIN and jing SU},
title = {Slightly acidic electrolyzed water cytotoxicity to oral keratinocyte monolayers},
year = {2018},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {26},
number = {6},
pages = {360-364},
keywords = {Dental unit waterlines, Slightly acidic electrolyzed water, Cytotoxicity, CCK-8 assay, TR146 cell line},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2018.06.004},
doi = {10.12016/j.issn.2096-1456.2018.06.004},
abstract = {ObjectiveTo investigate the cytotoxicity of slightly acidic electrolyzed water (SAEW) on oral keratinocyte monolayers.MethodsTR146 human keratinocyte monolayers were exposed to SAEW pretreated with bovine serum albumin (BSA). It was divided into 4 groups, BSA 0 mg/mL (SAEW stock solutsion), BSA 0.5 mg/mL, BSA 1 mg/mL and BSA 2 mg/mL. The relative growth rate (RGR) was measured using a CCK-8 assay at 1 min, 5 min, 15 min, 30 min and 1 h, and the survival rate was measured using a Trypan Blue exclusion assay at 1 h.ResultsThe CCK-8 assay showed significantly different OD values in the SAEW and negative control groups at different times and FAC concentrations (P<0.05). With increasing FAC concentrations and observation times, the RGR in the SAEW group decreased, and the SAEW showed moderate to severe cytotoxic effects. The OD values in the BSA (0.5~2 mg/mL)-pretreated SAEW and negative control groups were not significantly different at different times or FAC concentrations (P > 0.05); the RGRs of the BSA-pretreated SAEW group all approached 100%, and no cytotoxic effects were observed in the BSA-pretreated SAEW group. The Trypan Blue exclusion assay showed significantly different survival rates in the SAEW and negative control groups at different FAC concentrations (P < 0.05). As the FAC concentration increased, the survival rate in the SAEW group decreased, and SAEW showed moderate to severe cytotoxic effects. The survival rates in the BSA-pretreated SAEW and negative control groups were not significantly different at different FAC concentrations (P > 0.05); the survival rates in the BSA-pretreated SAEW group all approached 100%, and no cytotoxic effects were observed.ConclusionSAEW showed no adverse effects on the viability of dental oral keratinocyte monolayers in vitro in the presence of BSA at concentrations equivalent to that of protein in saliva.}
}