@article{YOU2017, 
author = {Xiaoqing YOU and Kai LUO and Yanfen LI and Chunfang WU and Fuhua YAN},
title = {Effect of Porphyromonas gingivalis lipopolysaccharide on gene expression of inflammatory cytokines in peritoneal macrophages of hyperlipidemic rabbits},
year = {2017},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {25},
number = {11},
pages = {687-692},
keywords = {Porphyromonas gingivalis, Lipopolysaccharide, Hyperlipidemia, Peritoneal macrophage, Inflammation factor, Periodontitis},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2017.11.002},
doi = {10.12016/j.issn.2096-1456.2017.11.002},
abstract = {ObjectiveTo compare the inflammatory reaction of peritoneal macrophage after Pg-LPS stimulated in healthy rabbit and hyperlipidemia rabbit.Methods12 New Zealand rabbits were randomly divided into 2 groups with 6 rabbits in each group, and normal diet and high-fat diet were fed to them respectively. The hyperlipidemia model was set up after 6 weeks. The peritoneal macrophage in normal and hyperlipidemia group were isolated and cultured, and then the cells in both groups were respectively divided into 3 groups: control, 1 μg/mL Pg-LPS, and 1 μg/mL E.coli-LPS. After 24 h treatment, the expressions of C-reaction protein (CRP), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) were detected by realtime PCR.ResultsThe levels of CRP, IL-1β, IL-6, IL-8, and TNF-α were higher in hyperlipidemia control group than normal control group. The expressions of inflammatory substances were increased after stimulated by Pg-LPS, and statistically higher in hyperlipidemia rabbit group than normal group (P < 0.05).ConclusionPg-LPS can enhance the mRNA expressions of inflammation related factors in peritoneal macrophage in hyperlipidemia rabbit.}
}