@article{HE2017, 
author = {Fei HE and Yuyan ZHENG and Wei CHOU and Guoquan ZHANG and Sui MAI},
title = {Effect of Notch signaling and autophagy on MTA induced differentiation of human dental pulp cells in vitro},
year = {2017},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {25},
number = {7},
pages = {414-419},
keywords = {Mineral trioxide aggregate, Dental pulp cells (DPCs), Differentiation, Notch, Autophagy},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2017.07.002},
doi = {10.12016/j.issn.2096-1456.2017.07.002},
abstract = {ObjectiveThe aim of this study is to investigate the roles of Notch signaling and autophagy on mineral trioxide aggregate (MTA) induced differentiation of human dental pulp cells (hDPCs).MethodsThird molars from healthy human were collected and hDPCs were isolated by a combined digestion of collagenase Ⅰ and dispaseⅡ. Real time PCR were used to test the mRNA expression levels of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2) and dentin sialophoprotein (DSPP) in MTA treated hDPCs in different time (24 h, 3 d and 7 d). The mineralization nodules formed by hDPCs with or without MTA treatment were detected by Von Kossa staining. Expressions of Notch1, Jagged1, Hes1, LC3Ⅱ/LC3 Ⅰand p62 in wild type and MTA treated hDPCs were detected by western blotting.ResultsMTA extracted in a concentration of 0.1 mg/mL could promote the differentiation of hDPCs. Compared with that of wild type hDPCs, the expressions of Notch1, Hes1, or Jagged1 and p62 (P＜0.01) in MTA treated hDPCs were significantly increased. MTA treatment showed inhibition effects on autophagy flux similar to Bafilomycin A1, a specific inhibitor of fusion between autophagosomes and lysosomes.ConclusionMTA could promote hDPCs differentiation with highly relevant in stimulating Notch1-Jagged1-Hes1 signaling and inhibition of autophagy flux.}
}