@article{WANG2017, 
author = {Huan-ling WANG and Jian-ping ZHANG},
title = {Effects of arecoline on apoptosis of human periodontal ligament fibroblasts},
year = {2017},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {25},
number = {4},
pages = {223-226},
keywords = {Arecoline, Human periodontal ligament fibroblasts, Apoptosis, Inhibition, Protein expression},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2017.04.004},
doi = {10.12016/j.issn.2096-1456.2017.04.004},
abstract = {ObjectiveTo observe the effects of arecoline on the related protein expressions in the apoptosis of cultured human periodontal ligament fibroblasts (hPDLFs), such as p-JNK, p-p53, and Bcl-2.MethodshPDLFs were isolated from human periodontal ligament tissues and expanded in vitro. hPDLFs were treated with different concentrations of arecoline (0, 20, 40 and 80 μg/m L) for 12 h. The proliferative activities were evaluated by MTT. The expressions of p-JNK, p-p53, and Bcl-2 were determined by Western blot.ResultsArecoline inhibited cell proliferation and induced apoptosis protein. The protein level of Bcl-2 was decreased, while p-JNK and p-p53 were increased (P < 0.05). The protein expressions were in a concentration - dependent manner with arecoline.ConclusionIt demonstrates arecoline might contribute to the apoptosis of hPDLFs, and could destroy periodontal tissues.}
}