@article{WEN2016, 
author = {Jun WEN and Shuai-mei XU and Ying LIU},
title = {Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells},
year = {2016},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {24},
number = {8},
pages = {459-463},
keywords = {Dental pulp stem cells, Adipogenic differentiation, Wnt passway, XAV-939, Cell proliferation},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2016.08.005},
doi = {10.12016/j.issn.2096-1456.2016.08.005},
abstract = {ObjectiveTo evaluate the effect of Wnt inhibitor XAV-939 on the proliferation and adipogenic differentiation of dental pulp stem cells (DPSCs).MethodsThe effect of XAV-939 on the proliferation of DPSCs was analyzed by cell counting kit-8(CCK-8). The effect of XAV-939 on the adipogenic differentiation of DPSCs was analyzed by oil red staining. Quantificational real-time polymerase chain reaction (qRT-PCR) was used to analyze the gene expression of glycogen synthase kinase-3β(GSK3β) and β-catenin which were Wnt signaling marker genes, and peroxisome proliferator activated receptor-γ2 (PPARγ2) and CCAAT/enhancer binding protein α(C/EBPα) which were adipogenic marker genes.ResultsAfter 14 days, XAV-939 up-regulates expression of GSK3β、PPARγ2 and C/EBPα, while down-regulates β-catenin.ConclusionXAV-939 could promote the adipogenic differentiation of DPSCs by inhibiting Wnt signalling pathway.}
}