TY  - JOUR
AU  - WEN, Jun
AU  - XU, Shuai-mei
AU  - LIU, Ying
PY  - 2016
TI  - Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells
JO  - Journal of Prevention and Treatment for Stomatological Diseases
SN  - 2096-1456
SP  - 459
EP  - 463
VL  - 24
IS  - 8
AB  - ObjectiveTo evaluate the effect of Wnt inhibitor XAV-939 on the proliferation and adipogenic differentiation of dental pulp stem cells (DPSCs).MethodsThe effect of XAV-939 on the proliferation of DPSCs was analyzed by cell counting kit-8(CCK-8). The effect of XAV-939 on the adipogenic differentiation of DPSCs was analyzed by oil red staining. Quantificational real-time polymerase chain reaction (qRT-PCR) was used to analyze the gene expression of glycogen synthase kinase-3β(GSK3β) and β-catenin which were Wnt signaling marker genes, and peroxisome proliferator activated receptor-γ2 (PPARγ2) and CCAAT/enhancer binding protein α(C/EBPα) which were adipogenic marker genes.ResultsAfter 14 days, XAV-939 up-regulates expression of GSK3β、PPARγ2 and C/EBPα, while down-regulates β-catenin.ConclusionXAV-939 could promote the adipogenic differentiation of DPSCs by inhibiting Wnt signalling pathway.
UR  - https://doi.org/10.12016/j.issn.2096-1456.2016.08.005
DO  - 10.12016/j.issn.2096-1456.2016.08.005