@article{CHENG2016, 
author = {Yan-fei CHENG and Yong-rong ZHONG},
title = {Study of the cellular growth inhibited by tetrandrine with the expression of β-catenin in oral squamous cell carcinoma cell line},
year = {2016},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {24},
number = {8},
pages = {464-468},
keywords = {Tetrandrine, Oral squamous cell carcinoma, β-catenin, Cell growth, Cell culture},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2016.08.006},
doi = {10.12016/j.issn.2096-1456.2016.08.006},
abstract = {ObjectiveTo study the effect of tetrandrine (TET) on oral squamous cell carcinoma cell line Tca8113 and its influence against the expression of β-catenin.MethodsCCK-8 method was used to detect the effect of TET on Tca8113 by different dose and time. Western blot assay was used to detect the expression of β-catenin after treatment by TET meanwhile the distribution of the β-catenin treated by TET was detected by the immunofluorescence method.ResultsWith the increasing dose of TET, the inhibition rate against Tca8113 was raising (P＜0.05). The half maximal inhibitory concentration (IC50) value of TET against Tca8113 was 64 μmol/L. With this concentration, the inhibition rate against Tca8113 exhibited a time-dependent manner. The results of western blot revealed that the expression of β-catenin was significantly inhibited after treatment by TET (P＜0.05). The results of immunofluorescence method showed that the β-catenin protein mainly located at nucleus in Tca8113, while after treatment by TET, the β-catenin protein mainly located at cellular membrane and cytoplasm in a diffused distribution manner.ConclusionOur data suggest that TET can inhibit the cell growth of oral squamous cell carcinoma, and influence the cellular distribution of β-catenin protein in tumor cells.}
}