TY  - JOUR
AU  - CHENG, Yan-fei
AU  - ZHONG, Yong-rong
PY  - 2016
TI  - Study of the cellular growth inhibited by tetrandrine with the expression of β-catenin in oral squamous cell carcinoma cell line
JO  - Journal of Prevention and Treatment for Stomatological Diseases
SN  - 2096-1456
SP  - 464
EP  - 468
VL  - 24
IS  - 8
AB  - ObjectiveTo study the effect of tetrandrine (TET) on oral squamous cell carcinoma cell line Tca8113 and its influence against the expression of β-catenin.MethodsCCK-8 method was used to detect the effect of TET on Tca8113 by different dose and time. Western blot assay was used to detect the expression of β-catenin after treatment by TET meanwhile the distribution of the β-catenin treated by TET was detected by the immunofluorescence method.ResultsWith the increasing dose of TET, the inhibition rate against Tca8113 was raising (P＜0.05). The half maximal inhibitory concentration (IC50) value of TET against Tca8113 was 64 μmol/L. With this concentration, the inhibition rate against Tca8113 exhibited a time-dependent manner. The results of western blot revealed that the expression of β-catenin was significantly inhibited after treatment by TET (P＜0.05). The results of immunofluorescence method showed that the β-catenin protein mainly located at nucleus in Tca8113, while after treatment by TET, the β-catenin protein mainly located at cellular membrane and cytoplasm in a diffused distribution manner.ConclusionOur data suggest that TET can inhibit the cell growth of oral squamous cell carcinoma, and influence the cellular distribution of β-catenin protein in tumor cells.
UR  - https://doi.org/10.12016/j.issn.2096-1456.2016.08.006
DO  - 10.12016/j.issn.2096-1456.2016.08.006