@article{WU2016, 
author = {Li-xia WU and Li-li ZHANG and Yuan-qin WANG and Lei LI and Shan-shan LIANG},
title = {Effects of 4 kinds of dental metal alloy extraction on expression of inflanmatory cytokine in the inflamed periodontal tissue},
year = {2016},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {24},
number = {8},
pages = {449-453},
keywords = {Gingival fibroblast cell, Co-Cr alloy, Ti alloy, Ti, Au-Pd alloy},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2016.08.003},
doi = {10.12016/j.issn.2096-1456.2016.08.003},
abstract = {ObjectiveTo evaluate the inflammatory cytokine expressions of inflamed human gingival fibroblast cells (iHGFs) stimulated in 4 types of dental metal extractions.MethodsInflamed gingival tissues from 10 patients with periodontitis were collected, and primary iHGFs were cultured. The cells were stimulated by Co-Cr, Ti alloy, pure Ti and AuPd alloy extractions respectively, and DMEM was used as blank control. Protein productions of pro-inflammatory mediators and related mRNA expressions were measured with ELISA and RT - PCR. Statistics were tested with Wilcoxon matched-pairs test.ResultsELISA and RT-PCR results showed the protein and mRNA expressions of interleukin-6, interleukin-1β, tumor necrosis factor-α in Ni-Cr and Ti alloy groups were both higher than the control group (z = 2.803, P =0.005). However, there was no significant difference in pure Ti and Au-Pd groups compared with the control group (P＞0.05).ConclusionPure Ti and Au-Pd alloy have a better biocompatibility than Co-Cr and Ti alloy in inflamed periodontal tissues.}
}