TY  - JOUR
AU  - WU, Li-xia
AU  - ZHANG, Li-li
AU  - WANG, Yuan-qin
AU  - LI, Lei
AU  - LIANG, Shan-shan
PY  - 2016
TI  - Effects of 4 kinds of dental metal alloy extraction on expression of inflanmatory cytokine in the inflamed periodontal tissue
JO  - Journal of Prevention and Treatment for Stomatological Diseases
SN  - 2096-1456
SP  - 449
EP  - 453
VL  - 24
IS  - 8
AB  - ObjectiveTo evaluate the inflammatory cytokine expressions of inflamed human gingival fibroblast cells (iHGFs) stimulated in 4 types of dental metal extractions.MethodsInflamed gingival tissues from 10 patients with periodontitis were collected, and primary iHGFs were cultured. The cells were stimulated by Co-Cr, Ti alloy, pure Ti and AuPd alloy extractions respectively, and DMEM was used as blank control. Protein productions of pro-inflammatory mediators and related mRNA expressions were measured with ELISA and RT - PCR. Statistics were tested with Wilcoxon matched-pairs test.ResultsELISA and RT-PCR results showed the protein and mRNA expressions of interleukin-6, interleukin-1β, tumor necrosis factor-α in Ni-Cr and Ti alloy groups were both higher than the control group (z = 2.803, P =0.005). However, there was no significant difference in pure Ti and Au-Pd groups compared with the control group (P＞0.05).ConclusionPure Ti and Au-Pd alloy have a better biocompatibility than Co-Cr and Ti alloy in inflamed periodontal tissues.
UR  - https://doi.org/10.12016/j.issn.2096-1456.2016.08.003
DO  - 10.12016/j.issn.2096-1456.2016.08.003