@article{HAN2016, 
author = {Qian-qian HAN and Zhao LIU and Li JIANG and Hui-yi TANG and Xiao-na LI},
title = {Effects of LMK-235 on osteoblast/odontoblast differentiation in hPDLCs},
year = {2016},
journal = {Journal of Prevention and Treatment for Stomatological Diseases},
volume = {24},
number = {7},
pages = {390-394},
keywords = {Periodontal ligament cells, Osteoblastic differentiation, Odontoblast differentiation, Histone deacetylase, Cell proliferation},
url = {https://www.sciopen.com/article/10.12016/j.issn.2096-1456.2016.07.003},
doi = {10.12016/j.issn.2096-1456.2016.07.003},
abstract = {ObjectiveTo investigate the effects of type Ⅱ a histone deacetylase inhibitor LMK-235 during early osteoblast/odontoblast differentiation in hPDLCs.MethodshPDLCs were obtained by the collagenase digestion method. hPDLCs at the 3rd passage were treated with medium containing 10% fetal bovine serum mixed with different concentrations of LMK-235 (0, 50, 100, 250, 500 nmol/L), respectively. Proliferative capability of hPDLCs was tested by MTT and qRT-PCR was used to detect mRNA expression levels of Runx2, ALP and DMP-1 3 d later.ResultsMTT assay showed that cell proliferation in hPDLCs treated with 100 nmol/L LMK-235 was increased significantly compared with the control group (P＜0.05). The expression of Runx2 mRNA in the 100 nmol/L group was 1.77 times of the control groups (P＜0.05). The expressions of ALP mRNA in all the experimental groups were significantly higher than that in control groups (P＜0.05), and the expression in the 100 nmol/L groups was the highest. The expressions of DMP-1 mRNA in the 50 and 100 nmol/L groups were higher than the control groups (P＜0.05).ConclusionType Ⅱ a histone deacetylase inhibitor LMK-235 could accelerate cell proliferation in hPDLCs at the concentration of 100 nmol/L, and regulate early osteoblast/odontoblast differentiation by upregulating the mRNA expressions of Runx2, ALP and DMP-1.}
}