@article{Zhuo2025, 
author = {Chenjian Zhuo and Xin Dong and Xueya Zhao and Weiru Wu and Hao Zhou and Jing Feng and Lingbo Liu and Mingqian Feng and Chunjiang He and Yu Hou},
title = {Single-cell sequencing reveals the expansion and diversity of T cell subsets in the bone marrow microenvironment of chronic myeloid leukemia},
year = {2025},
journal = {Genes & Diseases},
volume = {12},
number = {5},
pages = {101626},
keywords = {Chronic myeloid leukemia, T cell receptor, Single-cell sequencing, T cell, Cell-cell communication},
url = {https://www.sciopen.com/article/10.1016/j.gendis.2025.101626},
doi = {10.1016/j.gendis.2025.101626},
abstract = {The immune microenvironment plays an important role in leukemia treatment. However, a specific single-cell profiling of the immune alteration in bone marrow of chronic myeloid leukemia (CML) patients is still lacking. We performed multi-level single-cell sequencing to systematically decipher the bone marrow T cell atlas of CML patients. The results exhibited extensive changes of T cells, including the decreased CD4 T cells and increased CD8 T cells in the CML bone marrow. Subpopulation analysis revealed a significant increase of CD8 terminal effector (TE) cells and a significant decrease of CD4 naïve T cells. T cell receptor sequencing showed that the overall diversity of the T cell receptor repertoire was reduced in CML, with the exception of the CD8 TE cell. In addition, CD8 TE cells were the main source of gene expression differences in CD8 T cells. Intercellular communication analysis revealed the altered interaction between CD8 TE and other non-T cells in CML, including neutrophil subtype, indicating the potential regulation of bone marrow microenvironment cells on CD8 TE dynamics. Collectively, our work characterises the alteration of T cell subsets in CML patients at multiple single-cell levels, providing a valuable resource for understanding the immune microenvironment and developing new immune strategies for CML therapy.}
}