@article{WANG2025, 
author = {Xukai WANG and Congying CHEN and Yifeng RANG and Xueman XIAO and Yinhuan LI and Junming WANG and Chunhong LIU},
title = {In Vitro Nephrotoxicity of Migrants from Polybutylene adipate-co-terephthalate-Modified Starch Composite Material},
year = {2025},
journal = {Food Science},
volume = {46},
number = {7},
pages = {183-194},
keywords = {polybutylene adipate-co-terephthalat-modified starch composite material, migration, kidney injury, oxidative stress and inflammation},
url = {https://www.sciopen.com/article/10.7506/spkx1002-6630-20240504-005},
doi = {10.7506/spkx1002-6630-20240504-005},
abstract = {To investigate the nephrotoxicity of polybutylene adipate-co-terephthalate (PBAT)-modified starch composite material, substances migrating to different food simulants (4% acetic acid, 10% ethanol, and 95% ethanol) from the composite material were prepared and evaluated for its toxicity in human renal proximal tubule epithelial (HK-2) cells by analyzing the adenosine monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway and the expression levels of silent information regulator sirtuin 1 (SIRT1), peroxisome proliferators-activated receptor γ coactivator-l alpha (PGC-1α), nuclear respiratory factor-1 (NRF1) and recombinant transcription factor A (TFAM). The results showed that the migrants increased the expression level of kidney injury molecule-1 (KIM-1), elevated inflammatory factor levels and decreased antioxidant factor levels in HK-2 cells. The migrants upregulated the p-AMPK/AMPK ratio in HK-2 cells, and downregulated the p-mTOR/mTOR ratio as well as the expression levels of SIRT1, PGC-1α, NRF1, and TFAM. In summary, the migrants from the composite material could induce oxidative stress and inflammation in HK-2 cells. Furthermore, it might lead to mitochondrial dysfunction through the AMPK signaling pathway, resulting in kidney damage and posing a potential threat to human health.}
}