TY  - JOUR
AU  - GAO, Peng
AU  - ZHANG, Shengmeng
AU  - SUN, Yuanwei
AU  - HUANG, Weiwei
AU  - QIU, Yibin
AU  - XU, Hong
AU  - ZHANG, Qi
AU  - LI, Sha
PY  - 2025
TI  - Efficient Synthesis of Sweet Protein Brazzein by Komagataella phaffii
JO  - Food Science
SN  - 1002-6630
SP  - 111
EP  - 119
VL  - 46
IS  - 17
AB  - In this study, the expression system of Komagataella phaffii GS115 was optimized for improved secretion of the sweet protein Brazzein. The recombinant strain K. phaffii Bra-1 was successfully obtained, which produced 90.2 mg/L of Brazzein in shake flask fermentation under 2% methanol, 0.08% biotin, 30 ℃ and initial pH 6.0. Selection and optimization of expression elements showed that the endogenous alcohol oxidase 1 (AOX1) promoter had the best expression performance. The αΔ57-70 signal peptide increased the expression of Brazzein to 183.2 mg/L, which was further increased to 209.3 mg/L by the co-expression regulatory element, disulfide bond isomerase (PDI). In a 5 L bioreactor, fed-batch fermentation at an initial OD600 of 100 and a constant pH of 5.0 yielded 1.4 g/L of Brazzein. This study lays the foundation for the large-scale production and application of Brazzein.
UR  - https://doi.org/10.7506/spkx1002-6630-20250311-087
DO  - 10.7506/spkx1002-6630-20250311-087