@article{ZHANG2023, 
author = {Yalun ZHANG and Chen CHEN and Hui WANG and Tonghe ZHANG and Shuang ZHANG and Xian LI and Yongbo LI},
title = {Development of Ready-to-Use Quality Control Samples for Rapid Detection of Escherichia coli in Meat and Meat Products},
year = {2023},
journal = {Meat Research},
volume = {37},
number = {3},
pages = {46-50},
keywords = {stability, homogeneity, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, Escherichia coli, qualitative control samples},
url = {https://www.sciopen.com/article/10.7506/rlyj1001-8123-20220919-127},
doi = {10.7506/rlyj1001-8123-20220919-127},
abstract = {This paper developed a ready-to-use quality control sample which can be directly used without secondary culture for the rapid detection of Escherichia coli in meat and meat products by matrix assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS). The sample was evaluated for homogeneity and stability. The results showed that the survival rate of the quality control sample prepared using the selected cryoprotectant was above 90%. The culture count result of the quality control sample (F = 0.567) was less than the critical value, suggesting good uniformity. The quality control sample was stable for 14 days at 37 and 25 ℃, and the resuscitation rates were 101.5% and 99.6% at −20 and 4 ℃ for 28 days, respectively. After 28 days of storage at 37 and −20 ℃, the results of MALDI-TOF-MS were consistent, indicating that the developed sample had good stability and met the requirements of quality control samples.}
}