@article{GUO2022, 
author = {Honglei GUO and Shengyun ZHOU and Yanjun CONG and Wenjie YAN},
title = {Effects of Tea Polyphenols on Conformation and Antigenicity of Bovine Milk αs1-Casein Allergen},
year = {2022},
journal = {Journal of Dairy Science and Technology},
volume = {45},
number = {4},
pages = {7-13},
keywords = {αs1-casein, epigallocatechin gallate, epigallocatechin, conformation, antigenicity},
url = {https://www.sciopen.com/article/10.7506/rykxyjs1671-5187-20220508-026},
doi = {10.7506/rykxyjs1671-5187-20220508-026},
abstract = {In order to investigate the effects of tea polyphenols on the conformation and antigenicity of αs1-casein, epigallocatechin gallate (EGCG) and epigallocatechin (EGC), tea polyphenols with high bioactivity, were used for grafting onto αs1-casein. The conformational changes of αs1-casein were studied by fluorescence, synchronous fluorescence and circular dichroism (CD) spectroscopy, and the changes in the antigenicity of αs1-casein were analyzed by indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and Western blot assay. The results showed that EGC and EGCG both quenched the fluorescence of αs1-casein, and affected the Tyr and Trp residues of αs1-casein. The α-helix and β-sheet contents of αs1-casein increased slightly, and the random coil content decreased slightly. The conformational change of αs1-casein resulted in a significant decrease in the antigenicity of αs1-casein. The effect of EGCG on the conformation and antigenicity of αs1-casein was stronger than that of EGC.}
}